A nucleosome assembly factor is a constituent of simian virus 40 minichromosomes.
نویسندگان
چکیده
منابع مشابه
Accessibility to topoisomerases I and II regulates the replication efficiency of simian virus 40 minichromosomes.
We determined the effects of chromatin structure on template accessibility to replication factors and used three different templates as substrates for simian virus 40 (SV40) DNA replication in vitro: native and salt-treated SV40 minichromosomes and protein-free SV40 DNA. Native minichromosomes contain histone H1 and numerous nonhistone proteins in addition to the core histones, whereas salt-tre...
متن کاملModification of simian virus 40 protein A.
The A protein of simian virus 40 is phosphorylated in both productive and transforming infection. The phosphorylated amino acid has been identified as serine and has been localized in a single tryptic peptide of the protein. Because the A protein synthesized in infection by A mutants is phosphorylated to the same extent and in the same peptide as in infection by wild-type virus, the functional ...
متن کاملA block in initiation of simian virus 40 assembly results in the accumulation of minichromosomes containing an exposed regulatory region ( chromatin structure / nucleosome assembly / DNA replication / temperature - sensitive mutant )
The initiation of simian virus 40 assembly is blocked at the nonpermissive temperature in cells infected with the viral capsid protein VP1 mutant tsC219. Greater than 95% of the minichromosomes isolated from these cells are accessible to cleavage by Bgl I and Sph I, which recognize the sequences near the viral replication origin and in the transcription enhancer elements, respectively. The acce...
متن کاملControl of simian virus 40 gene expression in adenovirus-simian virus 40 hybrid viruses. Synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virus.
The effect of interferon on simian virus 40 (SV40) and adenovirus 2 (Ad2) T antigen synthesis has been examined in cells infected with SV40, with Ad2, and with a nondefective Ad2-SV40 hybrid virus, Ad2(+)ND(4). The induction of SV40 T antigen by SV40 was highly sensitive to interferon, whereas the induction of Ad2 T-antigen by Ad2 was resistant. This difference in interferon sensitivity was als...
متن کاملCell-specific activity of the constituent elements of the simian virus 40 enhancer.
We have compared the activation of transcription from the early promoter of simian virus 40 brought about by the enhancer during transient expression in several cell lines. The enhancer stimulated RNA synthesis approximately 400-, 100-, 100-, and 300-fold in human HeLa cells, the mouse plasmacytoma B cells MPC11, and the nondifferentiated and retinoic acid-differentiated mouse embryonal carcino...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1993
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.13.2.1059